Hotspot

rps2-rpoC2

LSC · 16263-16488 · 226 bp

Back to Euphrasia

Pi 0.0501

MarkerSeek score 83.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 16
pi: 0.0501
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCATGACCAAAATGAACGCC	TCGAAGGGGAAATCGGAGAT	50.0	50.0	58.0	58.2	389-390 bp	1.0	85.6
rps2-rpoC2_p2	CCCATGACCAAAATGAACGC	TCGAAGGGGAAATCGGAGAT	50.0	50.0	58.0	58.2	390-391 bp	1.0	85.6
rps2-rpoC2_p3	CAGCAGCTCTTCCAAGTTGA	TCGAAGGGGAAATCGGAGAT	50.0	50.0	58.1	58.2	363-364 bp	1.0	84.9
rps2-rpoC2_p4	CCATGACCAAAATGAACGCC	TTCGAAGGGGAAATCGGAGA	50.0	50.0	58.0	58.4	390-391 bp	1.0	83.7
rps2-rpoC2_p5	CCCATGACCAAAATGAACGC	TTCGAAGGGGAAATCGGAGA	50.0	50.0	58.0	58.4	391-392 bp	1.0	83.7