Hotspot

atpH-atpI

LSC · 13627-14581 · 955 bp

Back to Euphrasia

Pi 0.0335

MarkerSeek score 65.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 47
pi: 0.0335
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCGGACCAAAGAAACGAAAG	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1919-1924 bp	1.0	87.0
atpH-atpI_p2	TCCCCCGTATTTTTCCCTCT	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1436-1441 bp	1.0	86.9
atpH-atpI_p3	GTCCCCCGTATTTTTCCCTC	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1437-1442 bp	1.0	86.8
atpH-atpI_p4	GAGTCCCCCGTATTTTTCCC	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1439-1444 bp	1.0	86.7
atpH-atpI_p5	TCCCCCGTATTTTTCCCTCT	TTGGCAAATAGGGGGTTTCC	50.0	50.0	58.0	58.1	1769-1774 bp	1.0	86.4