Hotspot

atpA-atpF

LSC · 11710-11786 · 77 bp

Back to Euphrasia

Pi 0.0952

MarkerSeek score 68.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 11
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTTGAAGTACGGTACCGGT	CTGGGGGCGATGAAAGAAAT	50.0	50.0	58.4	58.2	211 bp	1.0	83.3
atpA-atpF_p2	ACTTGAAGTACGGTACCGGT	AATATTGACATGCTGGGGGC	50.0	50.0	58.4	58.3	223 bp	1.0	82.6
atpA-atpF_p3	TCGCCTACTTGAAGTACGGT	CTGGGGGCGATGAAAGAAAT	50.0	50.0	58.5	58.2	217 bp	1.0	82.5
atpA-atpF_p4	TCGCCTACTTGAAGTACGGT	AATATTGACATGCTGGGGGC	50.0	50.0	58.5	58.3	229 bp	1.0	81.8
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	AGTTGTTTGACCACCGAGTT	50.0	45.0	58.4	57.6	265 bp	1.0	81.0