Hotspot

trnR-UCU-atpA

LSC · 10068-10185 · 118 bp

Back to Euphrasia

Pi 0.0678

MarkerSeek score 61.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 12
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CAAATCCTATTGGACGCA	CTGACCGGTTTCTACTTCAGG	44.4	52.4	52.3	58.0	168-169 bp	1.0	49.7
trnR-UCU-atpA_p2	CAAATCCTATTGGACGCA	CAGGAACAAACTGACCGGTT	44.4	50.0	52.3	58.3	178-179 bp	1.0	49.4
trnR-UCU-atpA_p3	CAAATCCTATTGGACGCA	AGGAACAAACTGACCGGTTT	44.4	45.0	52.3	57.6	177-178 bp	1.0	49.4
trnR-UCU-atpA_p4	CAAATCCTATTGGACGCA	GGAACAAACTGACCGGTTTC	44.4	50.0	52.3	57.2	176-177 bp	1.0	49.4
trnR-UCU-atpA_p5	CAAATCCTATTGGACGCA	TTCAGGAACAAACTGACCGG	44.4	50.0	52.3	58.0	180-181 bp	1.0	49.3