Hotspot

rps16-trnQ-UUG

LSC · 6192-7243 · 1052 bp

Back to Euphrasia

Pi 0.026

MarkerSeek score 73.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 40
pi: 0.026
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GTTGGCGTCGATTCGAAATT	45.0	45.0	58.1	57.5	1197-1207 bp	1.0	81.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TGTTGGCGTCGATTCGAAAT	45.0	45.0	58.1	58.6	1198-1208 bp	1.0	81.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1155-1165 bp	1.0	80.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTGTTGGCGTCGATTCGAAA	45.0	45.0	58.1	58.8	1199-1209 bp	1.0	79.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTTGTTGGCGTCGATTCGAA	45.0	45.0	58.1	58.8	1200-1210 bp	1.0	79.3