Hotspot

trnH-GUG-psbA

LSC · 79-400 · 322 bp

Back to Euphrasia

Pi 0.0405

MarkerSeek score 61.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 322
variable_sites: 13
pi: 0.0405
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	1015-1016 bp	1.0	84.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GTTATACAACGGCGGTCCTT	55.0	50.0	58.1	58.0	1088-1089 bp	1.0	84.3
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	1029-1030 bp	1.0	84.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	1028-1029 bp	1.0	84.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	1030-1031 bp	1.0	84.3