primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	79	400	1015-1016	1.000000	84.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GTTATACAACGGCGGTCCTT	20	20	55.0	50.0	58.1	58.0	0.0	13.2	0.143255	79	400	1088-1089	1.000000	84.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	79	400	1029-1030	1.000000	84.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	79	400	1028-1029	1.000000	84.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	79	400	1030-1031	1.000000	84.3
trnK-UUU_p1	trnK-UUU	1	TCCCAATACCTATCGAAATCG	TCGGATTGGATCTATACACCCT	21	22	42.9	45.5	55.0	57.9	0.0	0.0	6.121029	1711	4254	2730-2730	1.000000	45.2
trnK-UUU_p2	trnK-UUU	2	AGAAAATCTGGATTGCTATGCT	TCGGATTGGATCTATACACCCT	22	22	36.4	45.5	55.8	57.9	1.0	0.0	6.297250	1711	4254	2657-2657	1.000000	45.2
trnK-UUU_p3	trnK-UUU	3	TCCCAATACCTATCGAAATCG	TCGGATTGGATCTATACACCC	21	21	42.9	47.6	55.0	56.4	0.0	0.0	6.601867	1711	4254	2730-2730	1.000000	45.2
trnK-UUU_p4	trnK-UUU	4	TCCCAATACCTATCGAAATCG	CGGATTGGATCTATACACCCT	21	21	42.9	47.6	55.0	56.4	0.0	0.0	6.611352	1711	4254	2729-2729	1.000000	45.2
trnK-UUU_p5	trnK-UUU	5	CCCAATACCTATCGAAATCG	TCGGATTGGATCTATACACCCT	20	22	45.0	45.5	53.4	57.9	0.0	0.0	6.734154	1711	4254	2729-2729	1.000000	45.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GTTGGCGTCGATTCGAAATT	20	20	45.0	45.0	58.1	57.5	8.1	10.9	0.660010	6192	7243	1197-1207	1.000000	81.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TGTTGGCGTCGATTCGAAAT	20	20	45.0	45.0	58.1	58.6	8.1	10.9	0.704769	6192	7243	1198-1208	1.000000	81.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6192	7243	1155-1165	1.000000	80.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TTGTTGGCGTCGATTCGAAA	20	20	45.0	45.0	58.1	58.8	8.1	10.9	0.920561	6192	7243	1199-1209	1.000000	79.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTTGTTGGCGTCGATTCGAA	20	20	45.0	45.0	58.1	58.8	8.1	10.9	0.920561	6192	7243	1200-1210	1.000000	79.3
trnS-GCU_p1	trnS-GCU	1	CCCCGGACGTGAAGAATAAA	GGAACTTCCTGATTTGCGGA	20	20	50.0	50.0	57.6	58.2	0.7	0.0	0.568745	8416	8503	286-286	1.000000	81.4
trnS-GCU_p2	trnS-GCU	2	AACCCCGGACGTGAAGAATA	GGAACTTCCTGATTTGCGGA	20	20	50.0	50.0	58.7	58.2	0.7	0.0	0.909772	8416	8503	288-288	1.000000	78.7
trnS-GCU_p3	trnS-GCU	3	ACCCCGGACGTGAAGAATAA	GGAACTTCCTGATTTGCGGA	20	20	50.0	50.0	58.7	58.2	0.7	0.0	0.909772	8416	8503	287-287	1.000000	78.7
trnS-GCU_p4	trnS-GCU	4	TAACCCCGGACGTGAAGAAT	GGAACTTCCTGATTTGCGGA	20	20	50.0	50.0	58.7	58.2	0.7	0.0	0.909772	8416	8503	289-289	1.000000	78.7
trnS-GCU_p5	trnS-GCU	5	ACCCCGGACGTGAAGAATA	GGAACTTCCTGATTTGCGGA	19	20	52.6	50.0	58.0	58.2	0.7	0.0	1.198243	8416	8503	287-287	1.000000	76.4
trnG-UCC_p1	trnG-UCC	1	GCTCAAGACTCTCGGTTTGA	GGAATTGTATATTCAATGGTTCGCT	20	25	50.0	36.0	57.6	58.0	0.0	8.2	5.470056	9083	9816	943-943	1.000000	47.5
trnG-UCC_p2	trnG-UCC	2	AGCTCAAGACTCTCGGTTTG	GGAATTGTATATTCAATGGTTCGCT	20	25	50.0	36.0	57.6	58.0	0.0	8.2	5.476761	9083	9816	944-944	1.000000	47.5
trnG-UCC_p3	trnG-UCC	3	CCCCCTGAAAAAGCTCAAGA	GGAATTGTATATTCAATGGTTCGCT	20	25	50.0	36.0	57.7	58.0	0.0	8.2	5.322345	9083	9816	955-955	1.000000	47.4
trnG-UCC_p4	trnG-UCC	4	CCCCCTGAAAAAGCTCAAGA	GGAATTGTATATTCAATGGTTCGC	20	24	50.0	37.5	57.7	56.8	0.0	8.2	5.523887	9083	9816	955-955	1.000000	47.4
trnG-UCC_p5	trnG-UCC	5	CCCCCTGAAAAAGCTCAAGA	TGGGAATTGTATATTCAATGGTTCG	20	25	50.0	36.0	57.7	57.7	0.0	8.2	5.563585	9083	9816	957-957	1.000000	47.4
trnR-UCU_p1	trnR-UCU	1	ACGAAAAATCGGAATGAACG	AGCCTTTACTTGCTATCGAA	20	20	40.0	40.0	55.0	54.5	3.1	0.0	6.490423	9996	10067	135-135	1.000000	47.6
trnR-UCU_p2	trnR-UCU	2	ACGAAAAATCGGAATGAACG	AGCCTTTACTTGCTATCGAAA	20	21	40.0	38.1	55.0	55.3	3.1	0.0	6.647381	9996	10067	135-135	1.000000	47.6
trnR-UCU_p3	trnR-UCU	3	ACGAAAAATCGGAATGAACG	AAGCCTTTACTTGCTATCGA	20	20	40.0	40.0	55.0	54.5	3.1	0.0	6.490423	9996	10067	136-136	1.000000	47.5
trnR-UCU_p4	trnR-UCU	4	ACGAAAAATCGGAATGAACG	AAAGCCTTTACTTGCTATCGA	20	21	40.0	38.1	55.0	55.3	3.1	0.0	6.647381	9996	10067	137-137	1.000000	47.5
trnR-UCU_p5	trnR-UCU	5	ACGAAAAATCGGAATGAACG	AAGCCTTTACTTGCTATCGAA	20	21	40.0	38.1	55.0	55.3	3.1	0.0	6.647381	9996	10067	136-136	1.000000	47.5
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	CAAATCCTATTGGACGCA	CTGACCGGTTTCTACTTCAGG	18	21	44.4	52.4	52.3	58.0	0.0	9.4	8.731489	10068	10185	168-169	1.000000	49.7
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CAAATCCTATTGGACGCA	CAGGAACAAACTGACCGGTT	18	20	44.4	50.0	52.3	58.3	0.0	9.4	8.016756	10068	10185	178-179	1.000000	49.4
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	CAAATCCTATTGGACGCA	AGGAACAAACTGACCGGTTT	18	20	44.4	45.0	52.3	57.6	0.0	9.4	8.122556	10068	10185	177-178	1.000000	49.4
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	CAAATCCTATTGGACGCA	GGAACAAACTGACCGGTTTC	18	20	44.4	50.0	52.3	57.2	0.0	9.4	8.461141	10068	10185	176-177	1.000000	49.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CAAATCCTATTGGACGCA	TTCAGGAACAAACTGACCGG	18	20	44.4	50.0	52.3	58.0	0.0	0.0	7.730514	10068	10185	180-181	1.000000	49.3
atpA-atpF_p1	atpA-atpF	1	ACTTGAAGTACGGTACCGGT	CTGGGGGCGATGAAAGAAAT	20	20	50.0	50.0	58.4	58.2	35.9	0.0	0.611419	11710	11786	211-211	1.000000	83.3
atpA-atpF_p2	atpA-atpF	2	ACTTGAAGTACGGTACCGGT	AATATTGACATGCTGGGGGC	20	20	50.0	50.0	58.4	58.3	35.9	0.0	0.670836	11710	11786	223-223	1.000000	82.6
atpA-atpF_p3	atpA-atpF	3	TCGCCTACTTGAAGTACGGT	CTGGGGGCGATGAAAGAAAT	20	20	50.0	50.0	58.5	58.2	13.2	0.0	0.694194	11710	11786	217-217	1.000000	82.5
atpA-atpF_p4	atpA-atpF	4	TCGCCTACTTGAAGTACGGT	AATATTGACATGCTGGGGGC	20	20	50.0	50.0	58.5	58.3	13.2	0.0	0.753611	11710	11786	229-229	1.000000	81.8
atpA-atpF_p5	atpA-atpF	5	ACTTGAAGTACGGTACCGGT	AGTTGTTTGACCACCGAGTT	20	20	50.0	45.0	58.4	57.6	35.9	0.0	0.792487	11710	11786	265-265	1.000000	81.0
atpF_p1	atpF	1	CACCCGCCATTACTTCATCA	AGAGGGAAAAATACGGGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061487	11787	13028	1936-1939	1.000000	86.9
atpF_p2	atpF	2	ATTCGACTAATTCACCCGCC	AGAGGGAAAAATACGGGGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.075064	11787	13028	1948-1951	1.000000	86.8
atpF_p3	atpF	3	AACGCCGGGAAATAATACCC	AGAGGGAAAAATACGGGGGA	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.053048	11787	13028	2206-2209	1.000000	86.6
atpF_p4	atpF	4	AACGCCGGGAAATAATACCC	GAGGGAAAAATACGGGGGAC	20	20	50.0	55.0	58.0	58.0	2.2	0.0	0.076963	11787	13028	2205-2208	1.000000	86.4
atpF_p5	atpF	5	AACGCCGGGAAATAATACCC	GGGAAAAATACGGGGGACTC	20	20	50.0	55.0	58.0	58.0	2.2	0.0	0.076963	11787	13028	2203-2206	1.000000	86.4
atpF-atpH_p1	atpF-atpH	1	GAAAGAATCGGTTACATTTTTCA	AGAGGGAAAAATACGGGGGA	23	20	30.4	50.0	53.9	58.0	0.0	0.0	7.120593	13029	13380	488-488	1.000000	47.6
atpF-atpH_p2	atpF-atpH	2	GAAAGAATCGGTTACATTTTTCA	GAGGGAAAAATACGGGGGAC	23	20	30.4	55.0	53.9	58.0	0.0	0.0	7.144508	13029	13380	487-487	1.000000	47.6
atpF-atpH_p3	atpF-atpH	3	GAAAGAATCGGTTACATTTTTCA	GGGAAAAATACGGGGGACTC	23	20	30.4	55.0	53.9	58.0	0.0	0.0	7.144508	13029	13380	485-485	1.000000	47.6
atpF-atpH_p4	atpF-atpH	4	GAAAGAATCGGTTACATTTTTCA	CAGAGGGAAAAATACGGGGG	23	20	30.4	55.0	53.9	57.9	0.0	0.0	7.148846	13029	13380	489-489	1.000000	47.6
atpF-atpH_p5	atpF-atpH	5	GAAAGAATCGGTTACATTTTTCA	AGGGAAAAATACGGGGGACT	23	20	30.4	50.0	53.9	58.3	0.0	0.0	7.418573	13029	13380	486-486	1.000000	47.6
atpH-atpI_p1	atpH-atpI	1	GCGGACCAAAGAAACGAAAG	TGCTTTGGCTTTACTCACGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.070621	13627	14581	1919-1924	1.000000	87.0
atpH-atpI_p2	atpH-atpI	2	TCCCCCGTATTTTTCCCTCT	TGCTTTGGCTTTACTCACGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.062736	13627	14581	1436-1441	1.000000	86.9
atpH-atpI_p3	atpH-atpI	3	GTCCCCCGTATTTTTCCCTC	TGCTTTGGCTTTACTCACGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.086651	13627	14581	1437-1442	1.000000	86.8
atpH-atpI_p4	atpH-atpI	4	GAGTCCCCCGTATTTTTCCC	TGCTTTGGCTTTACTCACGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.086651	13627	14581	1439-1444	1.000000	86.7
atpH-atpI_p5	atpH-atpI	5	TCCCCCGTATTTTTCCCTCT	TTGGCAAATAGGGGGTTTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.078950	13627	14581	1769-1774	1.000000	86.4
rps2_p1	rps2	1	ACCGATTCCCAAAATCTATTGT	TCAGGGTTCCTCGTCTCTTT	22	20	36.4	50.0	55.8	58.0	0.0	0.0	4.251454	15552	16262	812-812	1.000000	52.8
rps2_p2	rps2	2	ACCGATTCCCAAAATCTATTGT	TTCAGGGTTCCTCGTCTCTT	22	20	36.4	50.0	55.8	58.0	0.0	0.0	4.251454	15552	16262	813-813	1.000000	52.8
rps2_p3	rps2	3	ACCGATTCCCAAAATCTATTGT	TTTCAGGGTTCCTCGTCTCT	22	20	36.4	50.0	55.8	58.0	0.0	0.0	4.251454	15552	16262	814-814	1.000000	52.8
rps2_p4	rps2	4	ACCGATTCCCAAAATCTATTGT	GTTTCAGGGTTCCTCGTCTC	22	20	36.4	55.0	55.8	57.9	0.0	0.0	4.322057	15552	16262	815-815	1.000000	52.3
rps2_p5	rps2	5	ACCGATTCCCAAAATCTATTGT	CAGTTTCAGGGTTCCTCGTC	22	20	36.4	55.0	55.8	58.2	0.0	0.0	4.443307	15552	16262	817-817	1.000000	51.3
rps2-rpoC2_p1	rps2-rpoC2	1	CCATGACCAAAATGAACGCC	TCGAAGGGGAAATCGGAGAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.215561	16263	16488	389-390	1.000000	85.6
rps2-rpoC2_p2	rps2-rpoC2	2	CCCATGACCAAAATGAACGC	TCGAAGGGGAAATCGGAGAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.215561	16263	16488	390-391	1.000000	85.6
rps2-rpoC2_p3	rps2-rpoC2	3	CAGCAGCTCTTCCAAGTTGA	TCGAAGGGGAAATCGGAGAT	20	20	50.0	50.0	58.1	58.2	3.9	0.0	0.322263	16263	16488	363-364	1.000000	84.9
rps2-rpoC2_p4	rps2-rpoC2	4	CCATGACCAAAATGAACGCC	TTCGAAGGGGAAATCGGAGA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441160	16263	16488	390-391	1.000000	83.7
rps2-rpoC2_p5	rps2-rpoC2	5	CCCATGACCAAAATGAACGC	TTCGAAGGGGAAATCGGAGA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441160	16263	16488	391-392	1.000000	83.7
rpoC2_p1	rpoC2	1	TGTGAGATTTTCCATCGTTT	GCTCTCTGCTAATGGATCGG	20	20	35.0	55.0	53.1	57.9	0.0	0.0	4.965966	17776	17895	203-203	1.000000	52.3
rpoC2_p2	rpoC2	2	TTGTGAGATTTTCCATCGTT	GCTCTCTGCTAATGGATCGG	20	20	35.0	55.0	53.1	57.9	0.0	0.0	4.965966	17776	17895	204-204	1.000000	52.3
rpoC2_p3	rpoC2	3	TGTGAGATTTTCCATCGTTT	AATGGATCGGATTGCACCAA	20	20	35.0	45.0	53.1	57.9	0.0	0.8	5.030909	17776	17895	193-193	1.000000	52.0
rpoC2_p4	rpoC2	4	TTGTGAGATTTTCCATCGTT	AATGGATCGGATTGCACCAA	20	20	35.0	45.0	53.1	57.9	0.0	0.8	5.030909	17776	17895	194-194	1.000000	52.0
rpoC2_p5	rpoC2	5	TTGTGAGATTTTCCATCGTTT	GCTCTCTGCTAATGGATCGG	21	20	33.3	55.0	54.0	57.9	0.0	0.0	5.059260	17776	17895	204-204	1.000000	52.0
rpoC1_p1	rpoC1	1	CTGCTGAGTACGATTCGACA	AGGAAGGAAGCTTAATCGGA	20	20	50.0	45.0	57.5	55.9	14.0	0.0	2.612705	20727	23594	2995-2995	1.000000	64.9
rpoC1_p2	rpoC1	2	TTCTCGATCTTGATTCGCGT	AGGAAGGAAGCTTAATCGGA	20	20	45.0	45.0	57.4	55.9	5.8	0.0	2.629959	20727	23594	2942-2942	1.000000	64.8
rpoC1_p3	rpoC1	3	CTGCTGAGTACGATTCGACA	AGGAAGGAAGCTTAATCGGAA	20	21	50.0	42.9	57.5	56.7	14.0	0.0	2.827616	20727	23594	2995-2995	1.000000	63.1
rpoC1_p4	rpoC1	4	TTCTCGATCTTGATTCGCGT	AGGAAGGAAGCTTAATCGGAA	20	21	45.0	42.9	57.4	56.7	5.8	0.0	2.844870	20727	23594	2942-2942	1.000000	63.0
rpoC1_p5	rpoC1	5	TCTGCTGAGTACGATTCGAC	AGGAAGGAAGCTTAATCGGA	20	20	50.0	45.0	57.2	55.9	14.0	0.0	2.895204	20727	23594	2996-2996	1.000000	62.6
trnC-GCA_p1	trnC-GCA	1	GCGCAAATCCACTCAAAACA	AAAGAGGGGGCTTGACTTTG	20	20	45.0	50.0	57.9	58.0	0.3	0.0	0.133816	27940	28010	637-637	0.666667	65.7
trnC-GCA_p2	trnC-GCA	2	GCGCAAATCCACTCAAAACA	CTTTGTGAAAGAGGGGGCTT	20	20	45.0	50.0	57.9	58.0	0.3	0.0	0.133816	27940	28010	644-644	0.666667	65.7
trnC-GCA_p3	trnC-GCA	3	GCGCAAATCCACTCAAAACA	AAGGGGATGGGGAACATGTA	20	20	45.0	50.0	57.9	58.0	0.3	0.0	0.138341	27940	28010	805-805	0.666667	65.7
trnC-GCA_p4	trnC-GCA	4	GCGCAAATCCACTCAAAACA	GGTGCAGAGTCAAAAAGGGA	20	20	45.0	50.0	57.9	58.0	0.3	0.0	0.151484	27940	28010	921-921	0.666667	65.5
trnC-GCA_p5	trnC-GCA	5	GCGCAAATCCACTCAAAACA	TCAACGGTGCAGAGTCAAAA	20	20	45.0	45.0	57.9	58.0	0.3	0.0	0.163999	27940	28010	926-926	0.666667	65.4