Hotspot

ndhF-trnL-UAG

SSC · 120571-122187 · 1617 bp

Back to Euphorbia

Pi 0.085

MarkerSeek score 73.60

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1617
variable_sites: 600
pi: 0.085
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	TAGGGCGTGTGAACCAAAAA	TCCACACAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1620-2942 bp	0.927536	88.6
ndhF-trnL-UAG_p2	AATTCGAAGCTGCCCGTTAT	TCCACACAAAAGAGCTGCAT	45.0	45.0	58.0	58.0	1726-2997 bp	0.869565	86.4
ndhF-trnL-UAG_p3	AATTCGAAGCTGCCCGTTAT	ATCCACACAAAAGAGCTGCA	45.0	45.0	58.0	58.0	1727-2998 bp	0.855072	85.8
ndhF-trnL-UAG_p4	TGTTGTCGCCGGAAAAAGTA	ATCCACACAAAAGAGCTGCA	45.0	45.0	58.0	58.0	1212-2534 bp	0.289855	63.1
ndhF-trnL-UAG_p5	TGTTGTCGCCGGAAAAAGTA	TCCACACAAAAGAGCTGCAT	45.0	45.0	58.0	58.0	1211-2533 bp	0.289855	63.1