Hotspot

accD-psaI

LSC · 63225-64514 · 1290 bp

Back to Euphorbia

Pi 0.0673

MarkerSeek score 76.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1290
variable_sites: 497
pi: 0.0673
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGGATTAACCGAGGCTG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1679-2423 bp	1.0	91.3
accD-psaI_p2	GACAGGATTAACCGAGGCTG	GTTCTGAGCGCCAACTCATA	55.0	50.0	58.1	58.0	2042-2782 bp	0.942029	89.1
accD-psaI_p3	GACAGGATTAACCGAGGCTG	TATGTTCTGAGCGCCAACTC	55.0	50.0	58.1	58.0	2045-2785 bp	0.942029	89.1
accD-psaI_p4	GACAGGATTAACCGAGGCTG	TTCTGAGCGCCAACTCATAC	55.0	50.0	58.1	58.0	2041-2781 bp	0.942029	89.1
accD-psaI_p5	AGTATGGGATCCGTCGTAGG	TTCTGAGCGCCAACTCATAC	55.0	50.0	58.1	58.0	1947-2687 bp	0.942029	88.9