Hotspot

psbZ-trnG-GCC

LSC · 39012-40466 · 1455 bp

Back to Euphorbia

Pi 0.0856
MarkerSeek score 76.00
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
1455
variable_sites
583
pi
0.0856
unique_haplotype_count
65
primer_available
yes
alignment_reliability
0.78
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGGTATCCTTAATTCTCTCATCTCT CGTCTTCTCCTTGGCAAAGA 36.0 50.0 56.3 57.8 580-1601 bp 0.985507 51.4
psbZ-trnG-GCC_p2 TCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 34.8 50.0 54.3 57.8 575-1596 bp 0.985507 51.4
psbZ-trnG-GCC_p3 AGGTATCCTTAATTCTCTCATCTC CGTCTTCTCCTTGGCAAAGA 37.5 50.0 55.0 57.8 580-1601 bp 0.985507 51.4
psbZ-trnG-GCC_p4 GGTATCCTTAATTCTCTCATCTCT CGTCTTCTCCTTGGCAAAGA 37.5 50.0 55.0 57.8 579-1600 bp 0.985507 51.4
psbZ-trnG-GCC_p5 AGGTATCCTTAATTCTCTCATCTCTT CGTCTTCTCCTTGGCAAAGA 34.6 50.0 57.0 57.8 580-1601 bp 0.985507 51.4