Hotspot

petN-psbM

LSC · 30439-31719 · 1281 bp

Back to Euphorbia

Pi 0.0403

MarkerSeek score 76.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 365
pi: 0.0403
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTTCCCCAGTTCAAATCCGG	ATCCCGAGTTTTTGCGAAGT	50.0	45.0	58.1	58.1	1404-1940 bp	0.985507	89.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ATCCCGAGTTTTTGCGAAGT	50.0	45.0	58.2	58.1	1023-1536 bp	0.985507	89.3
petN-psbM_p3	GCCTCATCAACAAAAGACGC	ATCCCGAGTTTTTGCGAAGT	50.0	45.0	58.0	58.1	1379-1915 bp	0.782609	82.2
petN-psbM_p4	GCCTCATCAACAAAAGACGC	TGTAAGTGGGGGAGAAAGGT	50.0	50.0	58.0	57.9	2142 bp	0.014493	39.3
petN-psbM_p5	TTTCCCCAGTTCAAATCCGG	TGTAAGTGGGGGAGAAAGGT	50.0	50.0	58.1	57.9	2167 bp	0.014493	38.9