Hotspot

rpoB-trnC-GCA

LSC · 28807-29985 · 1179 bp

Back to Euphorbia

Pi 0.0495

MarkerSeek score 83.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 377
pi: 0.0495
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGATCCAACTCTGAGCGGTA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	1417-1928 bp	0.855072	84.7
rpoB-trnC-GCA_p2	CCTCATTTCCATCCTCGAGC	GCGTCTTTTGTTGATGAGGC	55.0	50.0	58.1	58.0	1246-1504 bp	0.347826	64.7
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCCTCGA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	57.8	58.0	1248-1506 bp	0.347826	64.3
rpoB-trnC-GCA_p4	TTCTGTTTCCTACTCACGCG	GCGTCTTTTGTTGATGAGGC	50.0	50.0	57.9	58.0	1754-1987 bp	0.246377	61.1
rpoB-trnC-GCA_p5	TTCTGTTTCCTACTCACGCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1729-1962 bp	0.246377	60.7