Hotspot

atpH-atpI

LSC · 15426-16475 · 1050 bp

Back to Euphorbia

Pi 0.0414

MarkerSeek score 76.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 280
pi: 0.0414
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGTGACCCAGGAAAACGAA	GGGTGCACTTTTACCTTGGA	50.0	50.0	58.0	58.0	1844-2176 bp	0.971014	90.5
atpH-atpI_p2	CCTCAAATCAGTCCTTCCCG	GGGTGCACTTTTACCTTGGA	55.0	50.0	58.0	58.0	1502-1841 bp	0.898551	87.6
atpH-atpI_p3	TGGACTAAGAAGGGGGAAGG	GGGTGCACTTTTACCTTGGA	55.0	50.0	58.0	58.0	1553-1892 bp	0.898551	87.6
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	GGGTGCACTTTTACCTTGGA	45.0	50.0	58.0	58.0	1156-1494 bp	0.855072	85.8
atpH-atpI_p5	CCCGCAGTAGGTTATTGTCC	GGGTGCACTTTTACCTTGGA	55.0	50.0	58.1	58.0	1486-1823 bp	0.086957	52.2