Hotspot

trnS-GCU-trnG-UCC

LSC · 9401-9973 · 573 bp

Back to Euphorbia

Pi 0.079
MarkerSeek score 78.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
573
variable_sites
230
pi
0.079
unique_haplotype_count
63
primer_available
yes
alignment_reliability
0.84
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAGTCACACTTTTACCACT 50.0 42.9 58.0 57.2 543-731 bp 1.0 77.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAGTCACACTTTTACCACT 50.0 42.9 58.0 57.2 542-730 bp 1.0 77.4
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AACGAGTCACACTTTTACCACT 50.0 40.9 58.0 57.9 544-732 bp 1.0 74.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AACGAGTCACACTTTTACCACT 50.0 40.9 58.0 57.9 543-731 bp 1.0 74.9
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC ACGAGTCACACTTTTACCACT 50.0 42.9 58.4 57.2 545-733 bp 1.0 74.6