Hotspot

trnK-UUU-rps16

LSC · 4565-6532 · 1968 bp

Back to Euphorbia

Pi 0.0837

MarkerSeek score 75.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1968
variable_sites: 824
pi: 0.0837
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGTTCCGCACCAAGAATTCA	ATGGGAGATAGGCAAATGCG	45.0	50.0	58.0	58.1	2430-2917 bp	0.492754	70.7
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	ATGGGAGATAGGCAAATGCG	55.0	50.0	58.0	58.1	2328-2815 bp	0.463768	69.6
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	TTGGGACTCGAACAAATGGG	55.0	50.0	58.0	58.1	2345-2715 bp	0.086957	54.6
trnK-UUU-rps16_p4	TGTTCCGCACCAAGAATTCA	TTGGGACTCGAACAAATGGG	45.0	50.0	58.0	58.1	2462-2634 bp	0.072464	51.8
trnK-UUU-rps16_p5	AAACTCATCAGCGGATTCCC	TTGGGACTCGAACAAATGGG	50.0	50.0	58.0	58.1	2880-2987 bp	0.057971	45.9