primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TGTTCCGCACCAAGAATTCA	ATGGGAGATAGGCAAATGCG	20	20	45.0	50.0	58.0	58.1	8.4	0.0	0.124144	4565	6532	2430-2917	0.492754	70.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	ATGGGAGATAGGCAAATGCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.114145	4565	6532	2328-2815	0.463768	69.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCAGGATCAGTCGTGG	TTGGGACTCGAACAAATGGG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.111449	4565	6532	2345-2715	0.086957	54.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TGTTCCGCACCAAGAATTCA	TTGGGACTCGAACAAATGGG	20	20	45.0	50.0	58.0	58.1	8.4	0.0	0.121448	4565	6532	2462-2634	0.072464	51.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAACTCATCAGCGGATTCCC	TTGGGACTCGAACAAATGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.139836	4565	6532	2880-2987	0.057971	45.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAGTCACACTTTTACCACT	20	21	50.0	42.9	58.0	57.2	0.0	0.0	1.818587	9401	9973	543-731	1.000000	77.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAGTCACACTTTTACCACT	20	21	50.0	42.9	58.0	57.2	0.0	0.0	1.819975	9401	9973	542-730	1.000000	77.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AACGAGTCACACTTTTACCACT	20	22	50.0	40.9	58.0	57.9	0.0	0.0	2.137596	9401	9973	544-732	1.000000	74.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	AACGAGTCACACTTTTACCACT	20	22	50.0	40.9	58.0	57.9	0.0	0.0	2.138984	9401	9973	543-731	1.000000	74.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGCAATCCGACGCTTTAGTC	ACGAGTCACACTTTTACCACT	20	21	50.0	42.9	58.4	57.2	0.0	0.0	2.172428	9401	9973	545-733	1.000000	74.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAGAAAAATTGCGTCCAA	20	20	50.0	40.0	58.0	56.2	0.0	0.0	1.876361	10745	11620	666-1228	0.985507	76.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAGAAAAATTGCGTCCAA	20	20	55.0	40.0	58.1	56.2	0.0	0.0	1.905197	10745	11620	669-1231	0.985507	76.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10745	11620	619-1181	1.000000	75.3
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10745	11620	622-1184	1.000000	75.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AGCCTTCCAAGCTAACGATG	ATGGAGAAAAATTGCGTCCA	20	20	50.0	40.0	58.0	55.9	0.0	0.0	2.108559	10745	11620	667-1229	0.985507	74.6
atpH-atpI_p1	atpH-atpI	1	CAGTGACCCAGGAAAACGAA	GGGTGCACTTTTACCTTGGA	20	20	50.0	50.0	58.0	58.0	0.0	18.0	0.048500	15426	16475	1844-2176	0.971014	90.5
atpH-atpI_p2	atpH-atpI	2	CCTCAAATCAGTCCTTCCCG	GGGTGCACTTTTACCTTGGA	20	20	55.0	50.0	58.0	58.0	0.0	18.0	0.040132	15426	16475	1502-1841	0.898551	87.6
atpH-atpI_p3	atpH-atpI	3	TGGACTAAGAAGGGGGAAGG	GGGTGCACTTTTACCTTGGA	20	20	55.0	50.0	58.0	58.0	0.0	18.0	0.043449	15426	16475	1553-1892	0.898551	87.6
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	GGGTGCACTTTTACCTTGGA	20	20	45.0	50.0	58.0	58.0	1.4	18.0	0.048681	15426	16475	1156-1494	0.855072	85.8
atpH-atpI_p5	atpH-atpI	5	CCCGCAGTAGGTTATTGTCC	GGGTGCACTTTTACCTTGGA	20	20	55.0	50.0	58.1	58.0	0.0	18.0	0.060828	15426	16475	1486-1823	0.086957	52.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TGATCCAACTCTGAGCGGTA	GCGTCTTTTGTTGATGAGGC	20	20	50.0	50.0	58.2	58.0	4.2	0.0	0.183568	28807	29985	1417-1928	0.855072	84.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCTCATTTCCATCCTCGAGC	GCGTCTTTTGTTGATGAGGC	20	20	55.0	50.0	58.1	58.0	7.0	0.0	0.154575	28807	29985	1246-1504	0.347826	64.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATCCTCGA	GCGTCTTTTGTTGATGAGGC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.205582	28807	29985	1248-1506	0.347826	64.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCTGTTTCCTACTCACGCG	GCGTCTTTTGTTGATGAGGC	20	20	50.0	50.0	57.9	58.0	3.5	0.0	0.091222	28807	29985	1754-1987	0.246377	61.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCTGTTTCCTACTCACGCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.9	58.1	3.5	0.0	0.142454	28807	29985	1729-1962	0.246377	60.7
petN-psbM_p1	petN-psbM	1	TTTCCCCAGTTCAAATCCGG	ATCCCGAGTTTTTGCGAAGT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.189918	30439	31719	1404-1940	0.985507	89.9
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	ATCCCGAGTTTTTGCGAAGT	20	20	50.0	45.0	58.2	58.1	0.0	0.0	0.259139	30439	31719	1023-1536	0.985507	89.3
petN-psbM_p3	petN-psbM	3	GCCTCATCAACAAAAGACGC	ATCCCGAGTTTTTGCGAAGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.138687	30439	31719	1379-1915	0.782609	82.2
petN-psbM_p4	petN-psbM	4	GCCTCATCAACAAAAGACGC	TGTAAGTGGGGGAGAAAGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.158548	30439	31719	2142-2142	0.014493	39.3
petN-psbM_p5	petN-psbM	5	TTTCCCCAGTTCAAATCCGG	TGTAAGTGGGGGAGAAAGGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.209779	30439	31719	2167-2167	0.014493	38.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33929	35724	1702-2241	0.985507	88.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33929	35724	1705-2244	0.985507	88.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GAGCCCCTTTAACTCAGTGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	57.9	58.2	0.0	0.0	0.361412	33929	35724	1718-2257	0.985507	88.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	33929	35724	1701-2240	0.971014	87.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.480767	33929	35724	1704-2243	0.971014	87.0
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	AGGTATCCTTAATTCTCTCATCTCT	CGTCTTCTCCTTGGCAAAGA	25	20	36.0	50.0	56.3	57.8	0.0	1.1	6.827174	39012	40466	580-1601	0.985507	51.4
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCTTAATTCTCTCATCTCTTGA	CGTCTTCTCCTTGGCAAAGA	23	20	34.8	50.0	54.3	57.8	0.0	1.1	6.894900	39012	40466	575-1596	0.985507	51.4
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	AGGTATCCTTAATTCTCTCATCTC	CGTCTTCTCCTTGGCAAAGA	24	20	37.5	50.0	55.0	57.8	0.0	1.1	7.178777	39012	40466	580-1601	0.985507	51.4
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GGTATCCTTAATTCTCTCATCTCT	CGTCTTCTCCTTGGCAAAGA	24	20	37.5	50.0	55.0	57.8	0.0	1.1	7.178777	39012	40466	579-1600	0.985507	51.4
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	AGGTATCCTTAATTCTCTCATCTCTT	CGTCTTCTCCTTGGCAAAGA	26	20	34.6	50.0	57.0	57.8	0.0	1.1	7.197665	39012	40466	580-1601	0.985507	51.4
psaA-pafI_p1	psaA-pafI	1	AGAGTTGGCCAAAATGAGCA	GAATTGACTGCTGAGCCGTA	20	20	45.0	50.0	58.0	58.0	22.5	0.3	0.010497	45863	46943	1215-1643	1.000000	91.9
psaA-pafI_p2	psaA-pafI	2	ATGCCACTCAGCCAAAGAAA	GAATTGACTGCTGAGCCGTA	20	20	45.0	50.0	58.0	58.0	0.0	0.3	0.010497	45863	46943	1243-1671	1.000000	91.9
psaA-pafI_p3	psaA-pafI	3	AGAGTTGGCCAAAATGAGCA	CTGAGCCGTATGAGGTAGGA	20	20	45.0	55.0	58.0	58.0	22.5	0.0	0.017692	45863	46943	1205-1633	1.000000	91.9
psaA-pafI_p4	psaA-pafI	4	ATGCCACTCAGCCAAAGAAA	CTGAGCCGTATGAGGTAGGA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.017692	45863	46943	1233-1661	1.000000	91.9
psaA-pafI_p5	psaA-pafI	5	ACATGTACTTGATGTCCCGC	GAATTGACTGCTGAGCCGTA	20	20	50.0	50.0	58.0	58.0	15.1	0.3	0.034321	45863	46943	1630-2058	1.000000	91.7
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	19	20	52.6	55.0	58.1	57.9	14.7	0.0	1.270002	50340	50778	527-604	1.000000	81.8
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	19	20	52.6	55.0	58.1	58.2	14.7	0.0	1.295812	50340	50778	496-573	1.000000	81.6
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	59.1	57.9	0.0	0.0	1.225254	50340	50778	545-622	0.985507	81.6
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGTTAGTCCCGGTAAAGCCC	GATGGTCATCGGTTCGACTC	20	20	55.0	55.0	59.1	58.2	0.0	0.0	1.251064	50340	50778	514-591	0.985507	81.4
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	AGTTAGTCCCGGTAAAGCCC	ATTTCTGGTATGTGAGCCCG	20	20	55.0	50.0	59.1	57.7	0.0	0.0	1.424577	50340	50778	570-647	0.971014	79.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCCCATCGCAATAACATA	TAGTGCTCTCGGATCGGAAT	20	20	50.0	50.0	58.1	58.0	0.0	4.4	0.113053	54420	55632	2137-2752	0.913043	87.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ATTGTAACCAAGCATCGCCT	TAGTGCTCTCGGATCGGAAT	20	20	45.0	50.0	57.9	58.0	0.0	4.4	0.146110	54420	55632	1569-2184	0.913043	87.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGTTCAGGCATTTGCTCAT	TAGTGCTCTCGGATCGGAAT	20	20	45.0	50.0	58.1	58.0	2.1	4.4	0.089852	54420	55632	350-2697	0.130435	56.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	TGTATCACAAACCCAAGGGC	20	20	45.0	50.0	58.1	58.1	2.1	4.7	0.154641	54420	55632	796-2601	0.086957	53.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTTCAGGCATTTGCTCAT	CGGGGCAATTTAGGTATCCC	20	20	45.0	55.0	58.1	58.1	2.1	0.0	0.166875	54420	55632	2410-2429	0.057971	42.0
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	AATGTCCCTGACACCGACTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085520	60934	61724	1598-1718	0.942029	89.0
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	ATGTCCCTGACACCGACTAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085520	60934	61724	1597-1717	0.942029	89.0
rbcL-accD_p3	rbcL-accD	3	AATGAGGGACGTGATCTTGC	ATGTCCCTGACACCGACTAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.096817	60934	61724	1021-1141	0.927536	88.3
rbcL-accD_p4	rbcL-accD	4	AATGAGGGACGTGATCTTGC	TCGCTTTCACCACAAACGTA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.092560	60934	61724	1429-1501	0.086957	51.2
rbcL-accD_p5	rbcL-accD	5	TTGAATGCTACTGCGGGTAC	TCGCTTTCACCACAAACGTA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.081264	60934	61724	2005-2077	0.086957	49.5
accD-psaI_p1	accD-psaI	1	GACAGGATTAACCGAGGCTG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.085678	63225	64514	1679-2423	1.000000	91.3
accD-psaI_p2	accD-psaI	2	GACAGGATTAACCGAGGCTG	GTTCTGAGCGCCAACTCATA	20	20	55.0	50.0	58.1	58.0	0.0	2.3	0.068820	63225	64514	2042-2782	0.942029	89.1
accD-psaI_p3	accD-psaI	3	GACAGGATTAACCGAGGCTG	TATGTTCTGAGCGCCAACTC	20	20	55.0	50.0	58.1	58.0	0.0	2.3	0.068820	63225	64514	2045-2785	0.942029	89.1
accD-psaI_p4	accD-psaI	4	GACAGGATTAACCGAGGCTG	TTCTGAGCGCCAACTCATAC	20	20	55.0	50.0	58.1	58.0	0.0	2.3	0.068820	63225	64514	2041-2781	0.942029	89.1
accD-psaI_p5	accD-psaI	5	AGTATGGGATCCGTCGTAGG	TTCTGAGCGCCAACTCATAC	20	20	55.0	50.0	58.1	58.0	5.5	2.3	0.103155	63225	64514	1947-2687	0.942029	88.9
rpl16_p1	rpl16	1	ACACATCAATTCTCGGGCTC	AATTGCACGAGTCGAATGGA	20	20	50.0	45.0	58.0	57.9	0.0	21.5	0.123145	88348	89953	2070-2383	0.956522	89.3
rpl16_p2	rpl16	2	ACACATCAATTCTCGGGCTC	TTGCACGAGTCGAATGGATT	20	20	50.0	45.0	58.0	57.9	0.0	21.5	0.123145	88348	89953	2068-2381	0.956522	89.3
rpl16_p3	rpl16	3	CTGCTTCCTTGATCACAGCA	TTGCACGAGTCGAATGGATT	20	20	50.0	45.0	58.2	57.9	14.3	21.5	0.275949	88348	89953	2144-2457	0.985507	89.2
rpl16_p4	rpl16	4	AGCAACAATAACGTCACCGA	AATTGCACGAGTCGAATGGA	20	20	45.0	45.0	57.8	57.9	0.0	21.5	0.251140	88348	89953	2130-2443	0.942029	87.7
rpl16_p5	rpl16	5	AGCAACAATAACGTCACCGA	TTGCACGAGTCGAATGGATT	20	20	45.0	45.0	57.8	57.9	0.0	21.5	0.251140	88348	89953	2128-2441	0.942029	87.7
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	AGCGGTCGCATTTCTTCTTA	20	20	55.0	45.0	58.0	57.6	0.0	17.7	0.382415	117128	118294	1374-2638	0.898551	84.9
ycf1_p2	ycf1	2	GAGCTACTGAGGAACAACGG	CAATGGAGTCGGTGTTACGA	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.359658	117128	118294	1666-1815	0.086957	47.2
ycf1_p3	ycf1	3	GAGCTACTGAGGAACAACGG	TCAATGGAGTCGGTGTTACG	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.359658	117128	118294	1667-1816	0.086957	47.2
ycf1_p4	ycf1	4	CCCCTTTGGTGTCATTGACA	CAATGGAGTCGGTGTTACGA	20	20	50.0	50.0	58.0	57.6	15.4	0.0	0.363371	117128	118294	1461-1610	0.072464	42.6
ycf1_p5	ycf1	5	CCCCTTTGGTGTCATTGACA	TCAATGGAGTCGGTGTTACG	20	20	50.0	50.0	58.0	57.6	15.4	0.0	0.363371	117128	118294	1462-1611	0.072464	42.6
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	TAGGGCGTGTGAACCAAAAA	TCCACACAAAAGAGCTGCAT	20	20	45.0	45.0	57.9	58.0	0.0	1.0	0.068759	120571	122187	1620-2942	0.927536	88.6
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	AATTCGAAGCTGCCCGTTAT	TCCACACAAAAGAGCTGCAT	20	20	45.0	45.0	58.0	58.0	3.3	1.0	0.052914	120571	122187	1726-2997	0.869565	86.4
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	AATTCGAAGCTGCCCGTTAT	ATCCACACAAAAGAGCTGCA	20	20	45.0	45.0	58.0	58.0	3.3	1.0	0.052914	120571	122187	1727-2998	0.855072	85.8
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	TGTTGTCGCCGGAAAAAGTA	ATCCACACAAAAGAGCTGCA	20	20	45.0	45.0	58.0	58.0	5.0	1.0	0.063699	120571	122187	1212-2534	0.289855	63.1
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	TGTTGTCGCCGGAAAAAGTA	TCCACACAAAAGAGCTGCAT	20	20	45.0	45.0	58.0	58.0	5.0	1.0	0.063699	120571	122187	1211-2533	0.289855	63.1