Hotspot

ycf1

SSC · 121813-126894 · 5082 bp

Back to Eupatorium

Pi 0.0038

MarkerSeek score 40.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5082
variable_sites: 29
pi: 0.0038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATTTCCTTGAGACGGTCCC	TTGAATGGGCTGTATCGTGG	55.0	50.0	58.0	58.0	598 bp	1.0	80.7
ycf1_p2	GGATTTCCTTGAGACGGTCC	TTGAATGGGCTGTATCGTGG	55.0	50.0	58.0	58.0	599 bp	1.0	80.7
ycf1_p3	GGGATTTCCTTGAGACGGTC	TTGAATGGGCTGTATCGTGG	55.0	50.0	58.0	58.0	600 bp	1.0	80.7
ycf1_p4	GATTTCCTTGAGACGGTCCC	GCAGAATACCGTCACCCATT	55.0	50.0	58.0	58.0	1209 bp	1.0	80.3
ycf1_p5	GGATTTCCTTGAGACGGTCC	GCAGAATACCGTCACCCATT	55.0	50.0	58.0	58.0	1210 bp	1.0	80.3