Hotspot

clpP1

LSC · 68867-70903 · 2037 bp

Back to Eupatorium

Pi 0.00427

MarkerSeek score 40.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2037
variable_sites: 13
pi: 0.00427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	ATTGACGTGAAATCCAGGCT	AGACTTTGTTACGTTTCCACA	45.0	38.1	57.5	55.7	2122-2125 bp	1.0	50.2
clpP1_p2	ATTGACGTGAAATCCAGGCT	GACTTTGTTACGTTTCCACATCA	45.0	39.1	57.5	57.6	2121-2124 bp	1.0	49.4
clpP1_p3	ATTGACGTGAAATCCAGGCT	ACTTTGTTACGTTTCCACATCA	45.0	36.4	57.5	56.5	2120-2123 bp	1.0	48.7
clpP1_p4	ATTGACGTGAAATCCAGGCT	TGTTACGTTTCCACATCAAAGT	45.0	36.4	57.5	56.5	2116-2119 bp	1.0	48.7
clpP1_p5	ATTGACGTGAAATCCAGGCT	AAGACTTTGTTACGTTTCCACA	45.0	36.4	57.5	56.4	2123-2126 bp	1.0	48.2