Hotspot

rpl32-trnL-UAG

SSC · 111450-112179 · 730 bp

Back to Eupatorium

Pi 0.01
MarkerSeek score 65.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
730
variable_sites
11
pi
0.01
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGAAAGGATATGGGGCG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 863-870 bp 1.0 78.0
rpl32-trnL-UAG_p2 AAAGGAAAGGATATGGGGCG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 864-871 bp 1.0 78.0
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 845-852 bp 1.0 77.8
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 846-853 bp 1.0 77.8
rpl32-trnL-UAG_p5 AAAGGAAAGGATATGGGGCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 873-880 bp 1.0 77.1