Hotspot

atpB-rbcL

LSC · 53443-54190 · 748 bp

Back to Eupatorium

Pi 0.00713

MarkerSeek score 63.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 8
pi: 0.00713
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	907-909 bp	1.0	72.5
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	906-908 bp	1.0	72.2
atpB-rbcL_p3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	834-836 bp	1.0	71.9
atpB-rbcL_p4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	864-866 bp	1.0	71.8
atpB-rbcL_p5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	833-835 bp	1.0	71.6