Hotspot

ndhC-trnV

LSC · 49500-50443 · 944 bp

Back to Eupatorium

Pi 0.00641

MarkerSeek score 57.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 9
pi: 0.00641
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1081-1101 bp	1.0	78.9
ndhC-trnV_p2	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1072-1092 bp	1.0	77.6
ndhC-trnV_p3	TTCTCCGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1089-1109 bp	1.0	77.2
ndhC-trnV_p4	TTTCTCCGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1090-1110 bp	1.0	76.3
ndhC-trnV_p5	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1080-1100 bp	1.0	76.2