Hotspot

trnK-UUU-rps16

LSC · 4363-5171 · 809 bp

Back to Eupatorium

Pi 0.00865
MarkerSeek score 64.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
809
variable_sites
10
pi
0.00865
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGTATGGATGAATCCCTCGC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 999-1003 bp 1.0 77.8
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 943-947 bp 1.0 77.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 942-946 bp 1.0 77.3
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1028-1032 bp 1.0 76.7
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1027-1031 bp 1.0 76.7