Hotspot

ycf1

SSC · 126154-131784 · 5631 bp

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Pi 0.0198

MarkerSeek score 58.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 478
pi: 0.0198
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGAAATGGGCGATTCCATC	TGATGAATCCGACTTTCCATTGA	50.0	39.1	58.4	58.2	193-220 bp	1.0	60.7
ycf1_p2	TCGAAATGGGCGATTCCATC	TGATGAATCCGACTTTCCATTG	50.0	40.9	58.4	56.8	193-220 bp	1.0	60.1
ycf1_p3	TCGAAATGGGCGATTCCATC	TGAATCCGACTTTCCATTGA	50.0	40.0	58.4	54.7	190-217 bp	1.0	59.3
ycf1_p4	TCGAAATGGGCGATTCCATC	TGAATCCGACTTTCCATTGAA	50.0	38.1	58.4	55.5	190-217 bp	1.0	58.0
ycf1_p5	TCGAAATGGGCGATTCCATC	GATGAATCCGACTTTCCATTGA	50.0	40.9	58.4	56.5	192-219 bp	1.0	58.0