Hotspot

rps19

LSC · 85680-85958 · 279 bp

Back to Euonymus

Pi 0.00239

MarkerSeek score 26.00

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 5
pi: 0.00239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGTCCCATAATCCCCCACT	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1210-1736 bp	1.0	89.0
rps19_p2	TTGTCCCATAATCCCCCACT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1209-1735 bp	1.0	89.0
rps19_p3	TCGGATAACTTCGTCCTCGA	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	984-1524 bp	1.0	88.7
rps19_p4	TCGGATAACTTCGTCCTCGA	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	983-1523 bp	1.0	88.7
rps19_p5	TTGTCCCATAATCCCCCACT	GATGAAGGAACGGGAAGACC	50.0	55.0	58.0	58.0	1625-2151 bp	1.0	87.6