Hotspot

accD

LSC · 59273-60763 · 1491 bp

Back to Euonymus

Pi 0.014

MarkerSeek score 52.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1491
variable_sites: 76
pi: 0.014
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATCATGGATCGAGCCGAGTA	CGTTCTGACCGACAACTCAT	50.0	50.0	58.1	57.9	2551-2836 bp	1.0	87.9
accD_p2	TCATGGATCGAGCCGAGTAT	CGTTCTGACCGACAACTCAT	50.0	50.0	58.1	57.9	2550-2835 bp	1.0	87.9
accD_p3	TCATGGATCGAGCCGAGTAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	2183-2308 bp	1.0	87.4
accD_p4	GCGTGATACTTCCGGTTCTT	CGTTCTGACCGACAACTCAT	50.0	50.0	58.0	57.9	2292-2570 bp	1.0	86.9
accD_p5	GCGTGATACTTCCGGTTCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1968-2040 bp	1.0	86.6