Hotspot

ccsA-ndhD

SSC · 117436-117695 · 260 bp

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Pi 0.0617

MarkerSeek score 71.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 260
variable_sites: 45
pi: 0.0617
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCTATGCGGCCCTTTTATGT	GGACCGCGAGAGTTGTTTAT	50.0	50.0	58.0	58.0	904-928 bp	1.0	89.3
ccsA-ndhD_p2	CGGCCCTTTTATGTGGATCA	GGACCGCGAGAGTTGTTTAT	50.0	50.0	57.9	58.0	898-922 bp	1.0	89.2
ccsA-ndhD_p3	GGGTATTCTTTCGGGAGCAG	GGACCGCGAGAGTTGTTTAT	55.0	50.0	58.0	58.0	630-654 bp	0.933333	88.1
ccsA-ndhD_p4	CCTATGCGGCCCTTTTATGT	GCATATGGGTTGGTTCGGAT	50.0	50.0	58.0	58.0	1504-1528 bp	1.0	86.1
ccsA-ndhD_p5	GGGTATTCTTTCGGGAGCAG	GCATATGGGTTGGTTCGGAT	55.0	50.0	58.0	58.0	1230-1254 bp	0.933333	83.4