Hotspot

trnN-GUU-ndhF

IRb · 110963-113092 · 2130 bp

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Pi 0.0161

MarkerSeek score 59.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2130
variable_sites: 129
pi: 0.0161
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	GCTTTAGGTCTGGGCTCTTC	55.0	55.0	58.0	58.0	2699-2870 bp	1.0	85.2
trnN-GUU-ndhF_p2	CGGGTTACCCACTCTTAACG	GCTTTAGGTCTGGGCTCTTC	55.0	55.0	58.0	58.0	2768-2939 bp	0.933333	82.0
trnN-GUU-ndhF_p3	AAGACAGAGGGAACGGGTTA	GCTTTAGGTCTGGGCTCTTC	50.0	55.0	58.0	58.0	2781-2952 bp	0.933333	81.8
trnN-GUU-ndhF_p4	CGGGAGAATCAATGACTGCA	GCTTTAGGTCTGGGCTCTTC	50.0	55.0	58.0	58.0	2979-2996 bp	0.466667	62.4
trnN-GUU-ndhF_p5	TTGTGGACTCTCGGGAGAAT	GCTTTAGGTCTGGGCTCTTC	50.0	55.0	58.1	58.0	2990-2997 bp	0.4	59.2