Hotspot

rps19-rpl2

IRb · 85959-86023 · 65 bp

Back to Euonymus

Pi 0.0414

MarkerSeek score 80.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 65
variable_sites: 9
pi: 0.0414
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCCTTTGTGTTAAGCTT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	162-675 bp	1.0	87.7
rps19-rpl2_p2	TTCCGCCTTTGTGTTAAGCT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	163-676 bp	1.0	87.7
rps19-rpl2_p3	TCCGCCTTTGTGTTAAGCTT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	254-767 bp	1.0	86.9
rps19-rpl2_p4	TTCCGCCTTTGTGTTAAGCT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	255-768 bp	1.0	86.9
rps19-rpl2_p5	TCCGCCTTTGTGTTAAGCTT	CTGGGGTTATCCTGCACTTG	45.0	55.0	58.0	58.2	220-733 bp	1.0	85.8