Hotspot

psbE-petL

LSC · 66074-67288 · 1215 bp

Back to Euonymus

Pi 0.0228

MarkerSeek score 70.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1215
variable_sites: 108
pi: 0.0228
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CCGCTAGTAGAAAACCGAAA	50.0	45.0	57.9	55.5	1298-1359 bp	1.0	70.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CCGCTAGTAGAAAACCGAAA	50.0	45.0	57.8	55.5	1221-1282 bp	1.0	69.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	CCGCTAGTAGAAAACCGAAA	45.0	45.0	58.2	55.5	1307-1368 bp	1.0	69.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	CCGCTAGTAGAAAACCGAAA	50.0	45.0	58.2	55.5	1312-1373 bp	1.0	69.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	CCGCTAGTAGAAAACCGAAA	50.0	45.0	57.8	55.5	1306-1367 bp	1.0	69.2