Hotspot

trnT-UGU-trnL-UAA

LSC · 48194-49146 · 953 bp

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Pi 0.0231

MarkerSeek score 78.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 88
pi: 0.0231
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTCTAACCGCTGAGCTAAGC	CGTACCAAGGCTCAATCCAA	55.0	50.0	58.2	57.9	996-1211 bp	1.0	88.6
trnT-UGU-trnL-UAA_p2	CTCTAACCGCTGAGCTAAGC	GTTTCCGTACCAAGGCTCAA	55.0	50.0	58.2	58.1	1001-1216 bp	1.0	88.6
trnT-UGU-trnL-UAA_p3	CTCTAACCGCTGAGCTAAGC	ATTCCGCCATATCCCCCATA	55.0	50.0	58.2	58.0	955-1170 bp	0.866667	82.4
trnT-UGU-trnL-UAA_p4	CTCTAACCGCTGAGCTAAGC	TTCCGCCATATCCCCCATAT	55.0	50.0	58.2	58.0	954-1169 bp	0.866667	82.3
trnT-UGU-trnL-UAA_p5	CTCTAACCGCTGAGCTAAGC	CCGTAGCGTCTACCAATTCC	55.0	55.0	58.2	58.2	970-1185 bp	0.866667	82.0