Hotspot

trnT-GGU-psbD

LSC · 32253-33701 · 1449 bp

Back to Euonymus

Pi 0.0204
MarkerSeek score 68.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1449
variable_sites
113
pi
0.0204
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTCCTTAACC 55.0 55.0 58.1 57.9 1567-1675 bp 1.0 87.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTCCTTAACC 50.0 55.0 58.1 57.9 1570-1678 bp 1.0 87.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1575-1683 bp 1.0 86.4
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1578-1686 bp 1.0 86.2
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGAAACGGTCCCTCCTTAAC 55.0 55.0 58.1 58.3 1568-1676 bp 1.0 86.2