Hotspot

atpH-atpI

LSC · 13788-14974 · 1187 bp

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Pi 0.0158

MarkerSeek score 72.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1187
variable_sites: 79
pi: 0.0158
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATCACAGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1126-1301 bp	1.0	84.1
atpH-atpI_p2	ATCACAGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1125-1300 bp	1.0	84.0
atpH-atpI_p3	AATCACAGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1099-1274 bp	1.0	83.7
atpH-atpI_p4	ATCACAGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	57.9	57.4	1098-1273 bp	1.0	83.6
atpH-atpI_p5	AATAGAAGCAAGACCGACGG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.7	58.5	1159-1334 bp	1.0	82.5