Hotspot

psbI-trnS-GCU

LSC · 7581-7810 · 230 bp

Back to Euonymus

Pi 0.0264

MarkerSeek score 71.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 26
pi: 0.0264
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCCCGGACGCGAAGAATAA	GTTCTTCGTACCGAGGGTTC	50.0	55.0	59.3	58.0	274-346 bp	1.0	80.8
psbI-trnS-GCU_p2	AATCCCGGACGCGAAGAATA	TGGACTAAAGCGTCGGATTG	50.0	50.0	59.3	58.0	311-383 bp	1.0	80.1
psbI-trnS-GCU_p3	ATCCCGGACGCGAAGAATAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	59.3	58.0	310-382 bp	1.0	80.1
psbI-trnS-GCU_p4	AATCCCGGACGCGAAGAATA	GTGGACTAAAGCGTCGGATT	50.0	50.0	59.3	58.0	312-384 bp	1.0	80.1
psbI-trnS-GCU_p5	ATCCCGGACGCGAAGAATAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	59.3	58.0	311-383 bp	1.0	80.1