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trnK-UUU-rps16

LSC · 4448-5081 · 634 bp

Back to Euonymus

Pi 0.0297

MarkerSeek score 69.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 68
pi: 0.0297
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTCATCTCCACCCCCTAA	CGATCGCGAAGAGAAGGAAA	55.0	50.0	58.1	58.1	1281-1355 bp	1.0	88.5
trnK-UUU-rps16_p2	CCTTCATCTCCACCCCCTAA	TTAGAACCCCCAACCCTCAT	55.0	50.0	58.1	57.9	1655-1738 bp	0.933333	85.5
trnK-UUU-rps16_p3	GCATTTTGTTTTGCGCCAAG	CGATCGCGAAGAGAAGGAAA	45.0	50.0	58.0	58.1	1045-1101 bp	0.866667	83.9
trnK-UUU-rps16_p4	GCATTTTGTTTTGCGCCAAG	TTAGAACCCCCAACCCTCAT	45.0	50.0	58.0	57.9	1409-1487 bp	0.8	80.7
trnK-UUU-rps16_p5	GCATTTTGTTTTGCGCCAAG	TTTAGAACCCCCAACCCTCA	45.0	50.0	58.0	58.2	1410-1488 bp	0.8	79.9