Hotspot

trnH-GUG-psbA

LSC · 74-551 · 478 bp

Back to Euonymus

Pi 0.0722

MarkerSeek score 71.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 101
pi: 0.0722
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACAATCCACTGCCTT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	505-693 bp	0.933333	88.0
trnH-GUG-psbA_p2	GATCCACAATCCACTGCCTT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	841-1029 bp	0.933333	87.9
trnH-GUG-psbA_p3	GATCCACAATCCACTGCCTT	ATTTCTTCCTAGCCGCTTGG	50.0	50.0	57.9	58.0	658-846 bp	0.933333	87.9
trnH-GUG-psbA_p4	GATCCACAATCCACTGCCTT	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	840-1028 bp	0.933333	87.9
trnH-GUG-psbA_p5	ATCCACAATCCACTGCCTTG	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	504-692 bp	0.933333	87.8