Hotspot

rpl16

LSC · 81681-83204 · 1524 bp

Back to Eulophia

Pi 0.0122

MarkerSeek score 63.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 18
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAGATAAAGAAATCCTTGGT	TCAAAGAAAAACGAACAATTAGA	37.5	26.1	57.4	53.2	1663-1681 bp	1.0	42.9
rpl16_p2	TCCAGAGATAAAGAAATCCTTGGT	TCTTAATCAAAGAAAAACGAACA	37.5	26.1	57.4	53.2	1669-1687 bp	1.0	42.9
rpl16_p3	TCCAGAGATAAAGAAATCCTTGGT	TCAAAGAAAAACGAACAATTAGAA	37.5	25.0	57.4	54.0	1663-1681 bp	1.0	42.9
rpl16_p4	TCCAGAGATAAAGAAATCCTTGGT	TCTTAATCAAAGAAAAACGAACAA	37.5	25.0	57.4	54.0	1669-1687 bp	1.0	42.9
rpl16_p5	TCAATGCAAGGAAGAATGAA	TCAAAGAAAAACGAACAATTAGA	35.0	26.1	53.1	53.2	1708-1721 bp	1.0	42.8