Hotspot

ndhA

SSC · 116147-117201 · 1055 bp

Back to Eulophia

Pi 0.0182

MarkerSeek score 56.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1055
variable_sites: 19
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCGACACAGTTACCGCAAAA	ATCACAAAGGGGTCTACGAA	45.0	45.0	58.1	56.2	1736 bp	0.5	44.8
ndhA_p2	TCGACACAGTTACCGCAAAA	TCACAAAGGGGTCTACGAAT	45.0	45.0	58.1	56.2	1735 bp	0.5	44.8
ndhA_p3	TGAACCAAGCAAGGGGTTTT	ATCACAAAGGGGTCTACGAA	45.0	45.0	58.1	56.2	1905 bp	0.5	44.3
ndhA_p4	TGAACCAAGCAAGGGGTTTT	TCACAAAGGGGTCTACGAAT	45.0	45.0	58.1	56.2	1904 bp	0.5	44.3
ndhA_p5	TTGAACCAAGCAAGGGGTTT	TCACAAAGGGGTCTACGAAT	45.0	45.0	58.1	56.2	1905 bp	0.5	44.3