Hotspot

ndhA-rps15

SSC · 117202-117288 · 87 bp

Back to Eulophia

Pi 0.0506

MarkerSeek score 72.90

Primer available yes

Label ndhA-rps15

Feature Metadata

label_name: ndhA-rps15
feature_type: igs
region: SSC
length_bp: 87
variable_sites: 4
pi: 0.0506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-rps15_p1	TGCATTTTATTTGTCTTGTTCCT	TTCTTGGAAAACGTCAACGA	30.4	40.0	55.3	55.9	219-227 bp	1.0	44.3
ndhA-rps15_p2	TGCATTTTATTTGTCTTGTTCCT	TCTTGGAAAACGTCAACGAT	30.4	40.0	55.3	55.6	218-226 bp	1.0	44.3
ndhA-rps15_p3	TTGCATTTTATTTGTCTTGTTCCT	TTCTTGGAAAACGTCAACGA	29.2	40.0	56.0	55.9	220-228 bp	1.0	44.3
ndhA-rps15_p4	TGCATTTTATTTGTCTTGTTCCT	TCTTGGAAAACGTCAACGATT	30.4	38.1	55.3	56.4	218-226 bp	1.0	44.3
ndhA-rps15_p5	TGCATTTTATTTGTCTTGTTCCT	TTCTTGGAAAACGTCAACGAT	30.4	38.1	55.3	56.4	219-227 bp	1.0	44.3