Hotspot

psaC-ndhE

SSC · 114294-114936 · 643 bp

Back to Eulophia

Pi 0.0272

MarkerSeek score 69.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 643
variable_sites: 16
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATACATCCGTGGGACAAGC	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.0	57.4	772-809 bp	1.0	80.3
psaC-ndhE_p2	AATACATCCGTGGGACAAGC	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.0	57.4	771-808 bp	1.0	80.3
psaC-ndhE_p3	AATACATCCGTGGGACAAGC	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.0	58.8	770-807 bp	1.0	79.2
psaC-ndhE_p4	AAGCTCGAACGCATTGAGTA	CTGAAGCAGCTATTGGGCTA	45.0	50.0	57.6	57.4	756-793 bp	1.0	77.7
psaC-ndhE_p5	AAGCTCGAACGCATTGAGTA	TGAAGCAGCTATTGGGCTAG	45.0	50.0	57.6	57.4	755-792 bp	1.0	77.7