Hotspot

ndhD-psaC

SSC · 113919-114047 · 129 bp

Back to Eulophia

Pi 0.0543

MarkerSeek score 70.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 129
variable_sites: 7
pi: 0.0543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGCCAAGGAAAATAACTCGTGA	CGCAGCATGGGTCTTTCTTA	40.9	50.0	58.0	58.3	176-186 bp	1.0	64.9
ndhD-psaC_p2	AGCCAAGGAAAATAACTCGTGA	CGCTTGTCCAACGGATTTTT	40.9	45.0	58.0	57.6	231-241 bp	1.0	62.9
ndhD-psaC_p3	AGCCAAGGAAAATAACTCGTGA	ATGAAACAACTCGCAGCATG	40.9	45.0	58.0	57.4	187-197 bp	1.0	61.8
ndhD-psaC_p4	AGCCAAGGAAAATAACTCGTGA	CATGAAACAACTCGCAGCAT	40.9	45.0	58.0	57.4	188-198 bp	1.0	61.8
ndhD-psaC_p5	GCCAAGGAAAATAACTCGTGA	CGCAGCATGGGTCTTTCTTA	42.9	50.0	56.5	58.3	175-185 bp	1.0	61.3