Hotspot

rps3-rpl22

LSC · 84040-84082 · 43 bp

Back to Eulophia

Pi 0.0465

MarkerSeek score 66.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 43
variable_sites: 2
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CGATACGTCCTGCAATTTGC	CATTTATTGGCGGGGGAGAA	50.0	50.0	57.9	57.9	939 bp	1.0	79.9
rps3-rpl22_p2	TCTTCGACTCCTCGTGTTCT	CATTTATTGGCGGGGGAGAA	50.0	50.0	58.1	57.9	715 bp	1.0	79.9
rps3-rpl22_p3	CGATACGTCCTGCAATTTGC	ATTTATTGGCGGGGGAGAAC	50.0	50.0	57.9	57.9	938 bp	1.0	79.8
rps3-rpl22_p4	CGATACGTCCTGCAATTTGC	CTCAACAGGAGGGAGGAGAA	50.0	55.0	57.9	58.1	1277 bp	0.5	58.8
rps3-rpl22_p5	TCTTCGACTCCTCGTGTTCT	CTCAACAGGAGGGAGGAGAA	50.0	55.0	58.1	58.1	1053 bp	0.5	58.7