Hotspot

rps8-rpl14

LSC · 80970-81177 · 208 bp

Back to Eulophia

Pi 0.0216

MarkerSeek score 67.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 4
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	GGTGCTATTGCTCGGGAATT	55.0	50.0	58.1	58.3	280-302 bp	1.0	81.7
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	CTATTGCTCGGGAATTGCGA	55.0	50.0	58.1	58.4	276-298 bp	1.0	80.9
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	TATTGCTCGGGAATTGCGAC	55.0	50.0	58.1	58.7	275-297 bp	1.0	78.6
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	GTGCTATTGCTCGGGAATTG	55.0	50.0	58.1	57.2	279-301 bp	1.0	78.1
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	TTTTGGTGCTATTGCTCGGG	55.0	50.0	58.1	58.8	284-306 bp	1.0	77.6