Hotspot

petD-rpoA

LSC · 78131-78311 · 181 bp

Back to Eulophia

Pi 0.0276

MarkerSeek score 68.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 5
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	ATTGGGCACTCTACAGAAGC	55.0	50.0	58.1	57.6	362 bp	1.0	77.0
petD-rpoA_p2	CGTCGTCCAGTAGCTACAAC	GGGCACTCTACAGAAGCATT	55.0	50.0	58.1	57.6	359 bp	1.0	77.0
petD-rpoA_p3	TTTTTGATCGGTACCGCAGT	ATTGGGCACTCTACAGAAGC	45.0	50.0	57.8	57.6	338 bp	1.0	76.5
petD-rpoA_p4	TTTTTGATCGGTACCGCAGT	GGGCACTCTACAGAAGCATT	45.0	50.0	57.8	57.6	335 bp	1.0	76.5
petD-rpoA_p5	GTACCGCAGTAGCTCTTTGG	GGGCACTCTACAGAAGCATT	55.0	50.0	58.4	57.6	325 bp	1.0	75.1