Hotspot

psbB-psbT

LSC · 73828-74427 · 600 bp

Back to Eulophia

Pi 0.0273
MarkerSeek score 56.50
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
600
variable_sites
9
pi
0.0273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.55
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT GGAATGTATAAACCAATGCTTCCA 45.0 37.5 57.7 57.5 460-729 bp 1.0 47.3
psbB-psbT_p2 TCAGAGATGTTTTTGCCGGT AGGAATGTATAAACCAATGCTTCC 45.0 37.5 57.7 57.3 461-730 bp 1.0 45.3
psbB-psbT_p3 GCCAGAACCTTGTTCAGAGA GGAATGTATAAACCAATGCTTCCA 50.0 37.5 57.4 57.5 473-742 bp 1.0 45.2
psbB-psbT_p4 GCCAGAACCTTGTTCAGAGA AGGAATGTATAAACCAATGCTTCC 50.0 37.5 57.4 57.3 474-743 bp 1.0 45.2
psbB-psbT_p5 TCAGAGATGTTTTTGCCGGT TCCCTAAAGTTGAAACTAAGAGGA 45.0 37.5 57.7 57.0 481-750 bp 1.0 45.2