Hotspot

trnW-CCA-trnP-UGG

LSC · 65834-66002 · 169 bp

Back to Eulophia

Pi 0.0373

MarkerSeek score 69.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 6
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	248-256 bp	1.0	83.0
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	247-255 bp	1.0	82.6
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	240-248 bp	1.0	82.6
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	275-283 bp	1.0	82.4
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	274-282 bp	1.0	81.9