Hotspot

trnF-GAA-ndhC

LSC · 48287-50184 · 1898 bp

Back to Eulophia

Pi 0.0149

MarkerSeek score 44.10

Primer available yes

Label trnF-GAA-ndhC

Feature Metadata

label_name: trnF-GAA-ndhC
feature_type: igs
region: LSC
length_bp: 1898
variable_sites: 6
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.21
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhC_p1	CCTCGTGTCACCAGTTCAAA	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.1	58.0	462-1957 bp	1.0	83.9
trnF-GAA-ndhC_p2	CCTCGTGTCACCAGTTCAAA	GTTCAGTCTATGCATGGCGA	50.0	50.0	58.1	58.1	481-1976 bp	1.0	83.5
trnF-GAA-ndhC_p3	AATGGTCGGGATAGCTCAGT	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.2	58.0	505-2000 bp	1.0	82.7
trnF-GAA-ndhC_p4	ATGGTCGGGATAGCTCAGTT	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.2	58.0	504-1999 bp	1.0	82.7
trnF-GAA-ndhC_p5	CGAAATGGTCGGGATAGCTC	AAAAGGAGCGTTGGAATGGT	55.0	45.0	58.2	58.0	508-2003 bp	1.0	82.6