Hotspot

psbM-trnD-GUC

LSC · 29258-30304 · 1047 bp

Back to Eulophia

Pi 0.0195

MarkerSeek score 64.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 20
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTGACTGTTTTGACGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1228-1231 bp	1.0	80.1
psbM-trnD-GUC_p2	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1206-1209 bp	1.0	80.0
psbM-trnD-GUC_p3	GCAGTGGCAATAAGAGCAAG	CCATGCATATGGCTGGGATT	50.0	50.0	57.4	58.1	1167-1170 bp	1.0	77.8
psbM-trnD-GUC_p4	GCAGTGGCAATAAGAGCAAG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.4	57.9	1145-1148 bp	1.0	77.7
psbM-trnD-GUC_p5	TGGCTGACTGTTTTGACGTA	CCATGCATATGGCTGGGATT	45.0	50.0	57.4	58.1	1227-1230 bp	1.0	77.3