Hotspot

rps16-trnQ-UUG

LSC · 5786-6774 · 989 bp

Back to Eulophia

Pi 0.019

MarkerSeek score 62.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 18
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GACCAAATCAAAATGGGGCG	50.0	50.0	57.2	58.0	1076-1116 bp	1.0	78.1
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	CAAATCAAAATGGGGCGTGG	50.0	50.0	57.2	58.3	1073-1113 bp	1.0	76.0
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	CCAAATCAAAATGGGGCGTG	50.0	50.0	57.2	58.3	1074-1114 bp	1.0	76.0
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGTTACTC	50.0	55.0	57.2	58.5	1036-1076 bp	1.0	74.5
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	ACCAAATCAAAATGGGGCGT	50.0	45.0	57.2	58.7	1075-1115 bp	1.0	72.9