Hotspot

trnK-UUU-rps16

LSC · 4066-4676 · 611 bp

Back to Eulophia

Pi 0.0136

MarkerSeek score 69.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 8
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGGATCAGTCGCGGTCTTAT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.3	58.7	763-789 bp	1.0	68.2
trnK-UUU-rps16_p2	AGGATCAGTCGCGGTCTTAT	TGCTCAACCCACAGGAACT	50.0	52.6	58.3	58.8	762-788 bp	1.0	67.4
trnK-UUU-rps16_p3	AGGATCAGTCGCGGTCTTAT	GTGCTCAACCCACAGGAACT	50.0	55.0	58.3	60.2	763-789 bp	1.0	64.1
trnK-UUU-rps16_p4	GTCGCAATTAAAAGCCGAGT	GTGCTCAACCCACAGGAAC	45.0	57.9	57.1	58.7	688-714 bp	1.0	63.9
trnK-UUU-rps16_p5	GTCGCAATTAAAAGCCGAGT	TGCTCAACCCACAGGAACT	45.0	52.6	57.1	58.8	687-713 bp	1.0	63.1