Hotspot

rpl32-trnL-UAG

SSC · 108116-108636 · 521 bp

Back to Eulalia

Pi 0.0272

MarkerSeek score 59.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 521
variable_sites: 14
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCTGGGCAACAAGGAAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.8	58.0	611-2926 bp	1.0	79.8
rpl32-trnL-UAG_p2	TTCTGGGCAACAAGGAAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.8	58.0	612-2925 bp	1.0	79.8
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.2	58.0	833-2926 bp	1.0	79.6
rpl32-trnL-UAG_p4	TCTGGGCAACAAGGAAACAA	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.8	58.0	1163-2926 bp	1.0	79.4
rpl32-trnL-UAG_p5	TTCTGGGCAACAAGGAAACA	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.8	58.0	1164-2925 bp	1.0	79.4